THE FREE AMINO ACID POOL OF CULTURED HUMAN CELLS

The intracellular free amino acid pool represents tracer precursor enrichment for calculation of protein synthesis in cultured fibroblasts and myocytes.

We assessed the approach of using intracellular free amino acid enrichment as precursor enrichment for calculating the fractional synthetic rate of using the stable isotope tracer incorporation technique. We assumed that the true rate of protein synthesis was reflected by the rate of tracer incorporation over time divided by the plateau enrichment in protein. Isolated human fibroblasts and myoc...

The amino acid pool in Escherichia coli.

The free amino acid pool of the haemocytes of Carcinus maenas (L.).

The functions of the blood cells of crustaceans have received little investigation. The majority of the work reported in the literature to date has been concerned with the involvement of the haemocytes in the phenomenon of plasma coagulation. In several crustacean species highly fragile blood cells have been described ' Hardy's explosive corpuscles' which are generally believed to contain subst...

A Study of the Properties of the Free Amino Acid Pool and Enzyme Synthesis in Yeast

A study has been made of the distribution and properties of the free amino acid pool in yeast. The depletion of the pool was found to depend upon the energy source used, conditions of growth, and the nature of the exogenous nitrogen source. Pool levels could be restored either by an internal replenishment mechanism or by various nitrogen sources. In the absence of internal replenishment a stron...

The stability of the free amino acid pool in isolated peripheral nerves of Carcinus maenas (L.).

Little information exists on the stability of the free amino acid pool of isolated peripheral nerves from the common shore crab, Carcinus maenas, when incubated in saline. The results of such a study would seem to be an essential prerequisite to the interpretation of influx and efflux rates obtained for selected amino acids using tracer techniques. Lewis (1954) observed that the rate of leakage...